HAMBATAN INVASI SEL KANKER LIDAH MANUSIA (SP-C1) MENGGUNAKAN EKSTRAK ETANOL DAUN KELADI TIKUS (Typhonium Hagelliforme Lodd.) in vitro

ABSTRACT

Ethanolic extract of Typhonium flagelliforme Lodd. had the potency of
growth inhibition of human cancer cell. The active compound that could inhibit the
growth of human cancer cell were saponin and phenolic. Saponin can be found most
in the leaf Some studies reported that the sitotoxic effect ethanolic extract of
Typhonium flagelliforme Lodd. detected inhibition some types of human cancer cell.
The aim of study was to examine the anti invasion potency ethanolic extract of
Typhonium flagelfiforme Lodd. leaves toward the growth inhibition of human oral
tongue cancer cell Supri's Clone-) (SP-Ct).

The design of this study was pure laboratory experimental research with the
sample of human oral tongue cancer cell (SP-C1). SP-C1 inyasion was inhibitad by
ethanolic extract of Typhonium flagelliforme Lodd. leaves in some concentrations (0,
25, 50, 75, 100, 125 ug/ml). The samples were ihen incubated in 24 hours. As a
control SP-C1 was grown in Rosswell Park Memorial Institute 1640 (RPMI-1640).
Boyden chamber kit were used in the study. Result of the study showed that ethanolic
extract of Typhonium flagelliforme Lodd. leaves was marked!y inhibit the invasion of
SP-C1 cell The conceniration of 125 ug/ml was found ethanolic exiract of
Typhonium flagelliforme Lodd. leaves has more to inhibit potency the invasion of
SP-C1 celi.

The conclusion ethanolic extract of Typhonium flagelliforme Lodd. leaves
has significant invasion to inhibit potency the invasion of human oral tongue cancer

cell line (SP-C1).

Key words: invasion, the human oral tongue cancer cell, Typhonium flagelliforme
Lodd. Icaves, Boyden chamber

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