PENELUSURAN AKTIVITAS ANTIKANKER FRAKSI ETANOL BIJI MELINJO (GNETUM GNEMON L.) TERHADAP SEL KANKER SERVIKS BERBASIS BIOINFORMATICS DAN UJI IN VITRO

The new cases of cervical cancer in Indonesia in 2020 reached 36,633 cases. Chemotherapy as a treatment strategy often causes adverse side effects. Melinjo seeds are known to contain compounds that have the potential as anticancer. This study aimed to investigate the anticancer activity of the ethanol fraction of melinjo seeds against cervical cancer cells based on in silico bioinformatics and in vitro assays. Melinjo seed powder was extracted using the maceration method, then fractionated to obtain MSEF. The identification test of resveratrol and oleic acid content in MSEF used the HPLC method. MSEF cytotoxic test against HeLa cells using the MTT Assay method with doxorubicin chemotherapy as a comparison. Cell migration test against MSEF and doxorubicin treatment as a comparison using the scratch wound healing assay method. STITCH-STRING bioinformatics as in silico test of resveratrol and oleic acid compounds to determine cancer target proteins. Molecular docking test to determine the binding affinity of resveratrol and oleic acid compounds to target proteins MYC, TP53, SERPINE1, and VEGFA with paclitaxel as a comparison. The results showed that MSEF was 61,02 grams, MSEF contained resveratrol compound, and didn’t contain oleic acid. MSEF has moderate cytotoxic activity against HeLa cells with an IC50 of 784 μg/mL. MSEF has inhibition of HeLa cell migration at IC50 FEBM concentration (784 μg/mL) with a migration rate of 0,61 μm/hour. Resveratrol compounds have interactions with cancer target proteins MYC and TP53, then oleic acid compounds with SERPINE1 and VEGFA proteins. The docking results obtained the best interaction with the strongest and most stable affinity for the compound resveratrol and protein TP53 with the lowest docking score of -7.2 kcal/mol. The results show that MSEF has the potential to be developed as a chemopreventive agent in cervical cancer