optimasi formulasi uji aktivitas antibakteri gel hand sanitizer kombinasi daun kemangi dan biji pepaya

Hand sanitizer is one of the antiseptic ingredients in the form of a gel that is often used by the public as a practical hand washing medium. The active ingredients of hand sanitizer preparations on the market are alcohol class compounds. A hand sanitizer that is made from nature or that contains natural ingredients is needed which is relatively safe when applied to the palms of the hands repeatedly. One of the plants that can replace alcohol and has the potential to be developed as an antiseptic is basil leaves (Ocimum sanctum L.) and papaya seeds (Carica papaya L.). This study aims to make a formulation and physical evaluation of hand sanitizer gel and to test the antibacterial activity of hand sanitizer gel, a combination of extracts of basil leaves and papaya seeds with three concentrations of 10%, 15% and 20% with HPMC as the base gel. This research used maceration method with 70% ethanol to extract the compound of basil leaves and papaya seeds. The results of the extraction were carried out by phytochemical tests on alkaloids, flavonoids, tannins, saponins, steroids and terpenoids. The hand sanitizer gel formulation uses a combination of basil leaf extract and papaya seeds as active compounds with concentrations of 10%, 15% and 20%. The hand sanitizer gel formulation was evaluated physically using the organoleptic test, pH, homogeneity, adhesion and spreadability. The results showed that the ethanol extract of basil leaves and papaya seeds contained alkaloids, falvonoids, tannins, saponins and terpenoids. Gel hand sanitizer combination of basil leaf extract and papaya seeds has inhibitory power against Escherichia coli with a concentration of 10%, 15% and 20%, and the average inhibition power is 6.33 mm, 8.66 mm and 11.55 mm, respectively. . The increase in the concentration of basil leaves and papaya seeds slightly affected the properties of the gel, namely organoleptic, homogeneity, pH, adhesion and dispersibility. The higher the concentration of basil leaves and papaya seeds, the stickiness and pH decreased, while the dispersion power, the antibacterial activity increased.