PENGUJIAN DETEKSI MOLEKULER JAMUR PATOGEN MELALUI DAUN PADA BERBAGAI KULTIVAR LOKAL SINGKONG

This study used native cassava cultivars from Gunungkidul to explore a method for detecting harmful fungi through diseased plant leaves. This study used molecular methods to identify and detect disease-causing fungi in diverse Gunungkidul cassava varieties, as well as descriptive methods to confirm the results of PCR detection. Morphological characteristics of pathogenic fungus, genomic quality and amount of cassava plant DNA, and PCR products are among the observation parameters. The ITS 1 Forward and ITS 4 Reverse primer pairs are used in the molecular detection treatment. Modifications were made by separating the DNA of infected cassava plants, electrophoresis was used to create excellent DNA bands, and the bands' results were then analyzed using a PCR test to see if the attacking disease's DNA was present. The mismatched primer pairs cause the expected product to not appear, which is 550 base pairs